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008 050307s2006 enka b 001 0 eng
010 |a  2005006606 
020 |a 1405111216 (pbk. : alk. paper) 
020 |a 9781405111218  |c (pbk. : alk. paper) 
035 |a (OCoLC)ocm58451766 
042 |a pcc 
082 0 0 |a 572.8/633  |2 22 
100 1 |a Brown, T. A. 
245 1 0 |a Gene cloning and DNA analysis :  |b an introduction /  |c T.A. Brown. 
250 |a 5th ed. 
260 |a Oxford ;  |a Malden, MA :  |b Blackwell Pub.,  |c 2006. 
300 |a xx, 386 p. :  |b ill. ;  |c 25 cm. 
504 |a Includes bibliographical references and index. 
505 0 |a PART 1: THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS: Why gene cloning and DNA are important -- Vectors for gene cloning: plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for E. coli -- Cloning vectors for eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- PART 2: THE APPLICATION OF GENE CLONING AND DNA ANALYSIS IN RESEARCH: Studying gene location and structure -- Studying gene expression and function -- Studying genomes -- PART 3: THE APPLICATION OF GENE CLONING AND DNA ANALYSIS IN BIOTECHNOLOGY: Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology -- Glossary. 
650 0 |a DNA  |x Analysis. 
650 0 |a Molecular cloning. 
650 0 |a Nucleotide sequence. 
650 1 2 |a Cloning, Molecular. 
650 2 2 |a DNA, Recombinant  |x analysis. 
650 2 2 |a Sequence Analysis, DNA. 
856 4 1 |u http://www.loc.gov/catdir/toc/ecip059/2005006606.html 
856 4 2 |u http://www.loc.gov/catdir/enhancements/fy0802/2005006606-b.html 
856 4 2 |u http://www.loc.gov/catdir/enhancements/fy0802/2005006606-d.html 
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