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Molecular Cloning: A Laboratory Manual
DSU Title III 2002-2012.
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| Other Authors: | |
| Format: | Printed Book |
| Published: |
New york
Cold Spring Harbor Laboratory Press
2001
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| Edition: | 3rd ed. |
| Subjects: |
Table of Contents:
- Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning
- Ch. 2. Bacteriophage and its vectors
- Ch. 3. Working with bacteriophage M13 vectors
- Ch. 4. Working with high-capacity vectors
- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
- Ch. 6. Preparation and analysis of eukaryotic genomic DNA
- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells
- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction
- Ch. 9. Preparation of radiolabeled DNA and RNA probes
- Ch. 10. Working with synthetic oligonucleotide probes
- Ch. 11. Preparation of cDNA libraries and gene identification
- Ch. 12. DNA sequencing
- Ch. 13. Mutagenesis
- Ch. 14. Screening expression libraries
- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli
- Ch. 16. Introducing cloned genes into cultured mammalian cells
- Ch. 17. Analysis of gene expression in cultured mammalian cells
- Ch. 18. Protein interaction technologies.