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Cloning Gene Expression and Protein purifiation
This combination lecture/laboratory manual presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties.
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| Format: | Printed Book |
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New York
Oxford University Press
2001
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Table of Contents:
- Introductory lecture
- Lecture DNA isolation
- Lecture 2 Constructionb of recombinant plasmids
- Lecture 3 The polymerase chain reaction
- Lecture 4 Transcription of genomic DNA and analysis of the resulting mRNA's
- Lecture 5 Transformation and gene expression
- Lecture 6 Analysis of DNA or RNA by duplex hybridization : DNA isolation, labeling, and probing
- Lecture 7 Protein purification
- Lecture 8 Discontinuos gel electrophoresis, protein mobilities, and apparent size determination
- Lecture 9 Immunochemical techniques
- Lecture 10 Combinatorial biochemical technology.