Cloning Gene Expression and Protein purifiation

This combination lecture/laboratory manual presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties.

Bibliographic Details
Main Author: Charles Hardin
Format: Printed Book
Published: New York Oxford University Press 2001
Subjects:
Molecular cloning > Laboratory manuals. Gene expression > Laboratory manuals. Proteins > Purification > Laboratory manuals.
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100 |a  Charles Hardin 
245 |a Cloning Gene Expression and Protein purifiation 
260 |a New York  |b Oxford University Press  |c 2001 
300 |a  viii, 435 pages : illustrations ; 28 cm 
505 |a  Introductory lecture -- Lecture DNA isolation -- Lecture 2 Constructionb of recombinant plasmids -- Lecture 3 The polymerase chain reaction -- Lecture 4 Transcription of genomic DNA and analysis of the resulting mRNA's -- Lecture 5 Transformation and gene expression -- Lecture 6 Analysis of DNA or RNA by duplex hybridization : DNA isolation, labeling, and probing -- Lecture 7 Protein purification -- Lecture 8 Discontinuos gel electrophoresis, protein mobilities, and apparent size determination -- Lecture 9 Immunochemical techniques -- Lecture 10 Combinatorial biochemical technology. 
520 |a This combination lecture/laboratory manual presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. 
650 |a  Molecular cloning -- Laboratory manuals. Gene expression -- Laboratory manuals. Proteins -- Purification -- Laboratory manuals.  
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