LC-MS/MS in proteomics : methods and applications /

Bibliographic Details
Other Authors: Cutillas, Pedro R., Timms, John F.
Format: Printed Book
Language:English
Published: New York, NY : Humana Press, c2010.
Series:Springer protocols
Methods in molecular biology, 658
Subjects:
Proteomics.
Liquid chromatography.
Tandem mass spectrometry.
Proteomics > methods > Laboratory Manuals.
Chromatography, Liquid > methods > Laboratory Manuals.
Tandem Mass Spectrometry > methods > Laboratory Manuals.
Computational Biology > methods > Laboratory Manuals.
Table of Contents:
  • Approaches and applications of quantitative LC-MS for proteomics and activitomics / Pedro R. Cutillas and John F. Timms
  • Overview of quantitative LC-MS techniques for proteomics and activitomics / John F. Timms and Pedro R. Cutillas
  • Instrumentation for LC-MS/MS in proteomics / Robert Chalkley
  • Bioinformatics for LC-MS/MS-based proteomics / Richard J. Jacob
  • Analysis of post-translational modifications by LC-MS/MS / Hannah Johnson and Claire E. Eyers
  • In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry / Maria P. Alcolea and Pedro R. Cutillas
  • Mapping of phosphorylation sites by LC-MS/MS / Bertran Gerrits and Bernd Bodenmiller
  • Analysis of carbohydrates on proteins by offline normal-phase liquid chromatography MALDI-TOF-MS/MS / Theodora Tryfona and Elaine Stephens
  • Selected reaction monitoring applied to quantitative proteomics / Reiko Kiyonami and Bruno Domon
  • Basic design of MRM assays for peptide quantification / Andrew James and Claus Jorgensen
  • Proteome-wide quantitation by SILAC / Kristoffer T.G. Rigbolt and Blagoy Blagoev
  • Quantification of proteins by iTRAQ / Richard D. Unwin
  • Quantification of proteins by label-free LC-MS/MS / Yishai Levin and Sabine Bahn
  • Protocol for quantitative proteomics of cellular membranes and membrane rafts / Andrew J. Thompson and Ritchie Williamson
  • Organelle proteomics by label-free and SILAC-based protein correlation profiling / Joern Dengjel, Lis Jakobsen, and Jens S. Andersen
  • Mapping protein-protein interactions by quantitative proteomics / Joern Dengjel, Irina Kratchmarova, and Blagoy Blagoev
  • Analysis of serum proteins by LC-MS/MS / Sarah Tonack, John P. Neoptolemos, and Eithne Costello
  • Urinary proteome profiling using 2D-DIGE and LC-MS/MS / Mark E. Weeks
  • Analysis of peptides in biological fluids by LC-MS/MS / Pedro R. Cutillas
  • Quantification of protein kinase activities by LC-MS / Maria P. Alcolea and Pedro R. Cutillas
  • A protocol for top-down proteomics using HPLC and ETD/PTR-MS / Sarah R. Hart.

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