লোডিং...
Proteomics in practice : a guide to successful experimental design /
| প্রধান লেখক: | |
|---|---|
| অন্যান্য লেখক: | , , |
| বিন্যাস: | Printed Book |
| ভাষা: | English |
| প্রকাশিত: |
Weinheim : Chichester :
Wiley-VCH ; John Wiley [distributor],
2008.
|
| সংস্করন: | 2nd ed. |
| বিষয়গুলি: |
সূচিপত্রের সারণি:
- Preface
- Foreword
- Abbreviations, symbols, units
- Introduction
- Pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions
- Pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search
- Pt. III. Trouble shooting
- Glossary of terms.