Proteomics in practice : a guide to successful experimental design /

Chi tiết về thư mục
Tác giả chính: Westermeier,Reiner
Tác giả khác: Westermeier, Reiner, Naven, Tom, Höpker, Hans-Rudolf
Định dạng: Printed Book
Ngôn ngữ:English
Được phát hành: Weinheim : Chichester : Wiley-VCH ; John Wiley [distributor], 2008.
Phiên bản:2nd ed.
Những chủ đề:
Proteins
Molecular biology.
Mục lục:
  • Preface
  • Foreword
  • Abbreviations, symbols, units
  • Introduction
  • Pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions
  • Pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search
  • Pt. III. Trouble shooting
  • Glossary of terms.

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