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02319cam a2200349 a 4500 |
| 008 |
080213s2008 gw a b 001 0 eng |
| 020 |
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|a 9783527319411 (cased)
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| 020 |
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|a 3527319417 (cased)
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| 035 |
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|a (OCoLC)ocn195752789
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| 035 |
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|a (OCoLC)195752789
|z (OCoLC)156819213
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|a (NNC)6633862
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|a ukblcatcopy
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|a 572.64
|2 22
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| 100 |
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|a Westermeier,Reiner
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| 245 |
0 |
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|a Proteomics in practice :
|b a guide to successful experimental design /
|c Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker.
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| 250 |
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|a 2nd ed.
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| 260 |
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|a Weinheim :
|b Wiley-VCH ;
|a Chichester :
|b John Wiley [distributor],
|c 2008.
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| 300 |
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|a xx, 482 p. :
|b ill. (some col.) ;
|c 25 cm.
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| 500 |
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|a Previous ed.: 2002.
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| 504 |
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|a Includes bibliographical references and index.
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| 505 |
0 |
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|a Preface -- Foreword -- Abbreviations, symbols, units -- Introduction -- Pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- Pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- Pt. III. Trouble shooting -- Glossary of terms.
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| 650 |
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|a Proteins
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| 650 |
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0 |
|a Molecular biology.
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| 700 |
1 |
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|a Westermeier, Reiner.
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| 700 |
1 |
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|a Naven, Tom.
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| 700 |
1 |
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|a Höpker, Hans-Rudolf.
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| 942 |
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|c REF
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| 900 |
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|a AUTH
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