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Methods in cell-matrix adhesion /
| Other Authors: | |
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| Format: | Printed Book |
| Language: | English |
| Published: |
Amsterdam : Boston :
Academic Press,
c2002.
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| Series: | Methods in cell biology ;
v. 69. |
| Subjects: |
Table of Contents:
- Preface and perspectives on cell-matrix adhesion--Matrix methodologies--Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies--Isolation and purification of proteoglycans--Expression of recombinant matrix components using baculoviruses--Heparan sulfate-growth factor interactions--Analysis of basement membrane self-assembly and cellular interactions with native and recombinant glycoproteins--Preparation and analysis of synthetic multicomponent extracellular matrix--Analysis of matrix dynamics by atomic force micrscopy--Analysis of matrix degradation--Adhesion receptor mathodologies--Expression cloning strategies for the identification of adhesion molecules--Purification of integrins and characterization of integrin-associated proteins--Methods for analysis of the integrin ligand binding event--Intracellular coupling of adhesion receptors--Functional applications of cell-matrix adhesion in molecular cell biology--Measurements of glycosaminoglycan-based cell interactions--Applications of adhesion molecule gene knockout cell lines--Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions--Cell migration in slice cultures--Application of cell adhesion to study signaling networks--Use of micropatterned adhesive surfaces for control of cell behavior--Adenoviral-mediated gene transfer in two-dimensional and three dimensional cultures of mammary epithelial cells.