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Methods in cell-matrix adhesion /

Bibliographic Details
Other Authors: Adams, Josephine C.
Format: Printed Book
Language:English
Published: Amsterdam : Boston : Academic Press, c2002.
Series:Methods in cell biology ; v. 69.
Subjects:
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245 0 0 |a Methods in cell-matrix adhesion /  |c Edited by Josephine C. Adams. 
260 |a Amsterdam :  |a Boston :  |b Academic Press,  |c c2002. 
300 |a xv, 461 p., [10] p. of plates :  |b ill. (some col.) ;  |c 25 cm. 
490 1 |a Methods in cell biology.  |x 0091-679x ;  |v v. 69 
500 |a Series originally edited by D.M. Prescott. 
505 |a Preface and perspectives on cell-matrix adhesion--Matrix methodologies--Detection and purification of instructive extracellular matrix components with monoclonal antibody technologies--Isolation and purification of proteoglycans--Expression of recombinant matrix components using baculoviruses--Heparan sulfate-growth factor interactions--Analysis of basement membrane self-assembly and cellular interactions with native and recombinant glycoproteins--Preparation and analysis of synthetic multicomponent extracellular matrix--Analysis of matrix dynamics by atomic force micrscopy--Analysis of matrix degradation--Adhesion receptor mathodologies--Expression cloning strategies for the identification of adhesion molecules--Purification of integrins and characterization of integrin-associated proteins--Methods for analysis of the integrin ligand binding event--Intracellular coupling of adhesion receptors--Functional applications of cell-matrix adhesion in molecular cell biology--Measurements of glycosaminoglycan-based cell interactions--Applications of adhesion molecule gene knockout cell lines--Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions--Cell migration in slice cultures--Application of cell adhesion to study signaling networks--Use of micropatterned adhesive surfaces for control of cell behavior--Adenoviral-mediated gene transfer in two-dimensional and three dimensional cultures of mammary epithelial cells. 
650 0 |a Cell adhesion. 
650 0 |a Extracellular matrix. 
700 1 |a Adams, Josephine C. 
942 |c BK 
830 0 |a Methods in cell biology ;  |v v. 69. 
900 |a AUTH 
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