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Advances in Molecular Techniques /
"Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists. This book serves as a guide that introduces classic molecular biology techniques a...
| Principais autores: | , , , |
|---|---|
| Formato: | Printed Book |
| Idioma: | English |
| Edição: | First edition. |
| Assuntos: | |
| Acesso em linha: | Taylor & Francis - Full text online |
| LEADER | 05357cam a2200577Mi 4500 | ||
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| 020 | |a 1351021222 | ||
| 020 | |a 9781351021203 | ||
| 020 | |a 9781351021210 | ||
| 020 | |a 9781351021227 | ||
| 020 | |z 081537075X | ||
| 020 | |z 9780815370758 | ||
| 035 | |a (OCoLC)on1031316327 | ||
| 049 | |a UIUU |o skip | ||
| 072 | 7 | |a MED009000 |2 bisacsh | |
| 072 | 7 | |a SCI029000 |2 bisacsh | |
| 072 | 7 | |a SCI049000 |2 bisacsh | |
| 082 | |a 572.33 | ||
| 100 | 1 | |a Sengar, Rakesh S., | |
| 245 | 1 | 0 | |a Advances in Molecular Techniques / |c Rakesh S. Sengar, Amit Kumar, Reshu Chaudhary, Ashu Singh. |
| 250 | |a First edition. | ||
| 300 | |a 436 P. | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Cover; Half Title; Title Page; Copyright Page; Dedication; Contents; Foreword; Preface; Authors; 1. DNA Manipulative and Ingratiative Techniques; Isolation of Total Genomic DNA; Isolation of Plant DNA Using the CTAB Method; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Isolation of Plant DNA Using the SDS Method; Introduction; Materials Required; Method; Precautions; To Isolate Plasmid DNA from Bacteria; Introduction; Materials Required; Method; Precautions; Isolation of Chloroplasts; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation. | |
| 505 | 8 | |a MethodResults; Precautions; Isolation of Chloroplast DNA; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Method; Results; Precautions; Isolation of Yeast Genomic DNA; Introduction; Materials Required; Method; Isolation of Bacterial Genomic DNA; Introduction; Materials Required; Method; Large-Scale Isolation of Cosmid DNA; Isolation; Purification; Miniprep Double-Stranded DNA Isolation; Principle; Isolation; Standard Alkaline Lysis Purification; Diatomaceous Earth-Base Purification; Plasmid Miniprep by Birnboim Method; Materials Required. | |
| 505 | 8 | |a Plasmid Miniprep by Boiling MethodMaterials Required; Method; Isolation of Lambda (.) DNA; Materials Required; Method; Quick Method for isolation of Lambda (.) DNA; Materials Required; Single-Stranded M13 DNA Isolation Using Phenol; Method; Biomek-Automated Modified-Eperon Isolation Procedure for Single-Stranded M13 DNA; Method; Isolation of Aspergillus DNA; Materials Required; Method; Isolation of Aspergillus DNA by the Qiagen Method; Materials Required; Method; Purification of Genomic DNA; Introduction; Principle; Materials Required; Method; Materials Required. | |
| 505 | 8 | |a Isolation of RNA from Plant TissuesIntroduction; Extraction of Total RNA; Materials Required; Method; Precautions; Extraction of Polysomal RNA of Nuclear Sequences; Materials Required; Chemicals; Glassware and Other; Equipment; Isolation of Total RNA form Bacterial Cells; Introduction; Materials Required; Method; Quantitative Determination of DNA and RNA by a Spectrophotometric Method; Introduction; Materials Required; Agarose Gel Electrophoresis; Introduction; Preparation of Agarose Gel; Electrophoresis of DNA Fragments; Visualization of DNA Fragments; Materials Required; Chemicals. | |
| 505 | 8 | |a Glassware and OtherEquipment; Electrophoresis of DNA: Linear, Circular, and Super Coiled; Introduction; Experimental Outline; Materials Required; Method; Precautions; Elution of Plant DNA Fragment from Agarose Gel; Introduction; Materials Required; Method; Precautions; Hybridization and Autoradiography of DNA; Introduction; Experimental Outline; Materials Required; Method; Hybridization; Autoradiography; Precautions; PCR in Outline; Introduction; Choice Between Cloning and PCR; Experimental Outline; Materials Required; Method; Precautions. | |
| 520 | 2 | |a "Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists. This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering."--Provided by publisher. | |
| 650 | 0 | |a Genetics. | |
| 650 | 0 | |a Molecular biology. | |
| 650 | 0 | |a Biotechnology. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Biotechnology. |2 fast | |
| 650 | 7 | |a Genetics. |2 fast | |
| 650 | 7 | |a Molecular biology. |2 fast | |
| 700 | 1 | |a Kumar, Amit, |e author. | |
| 700 | 1 | |a Chaudhary, Reshu, |e author. | |
| 700 | 1 | |a Singh, Ashu, |e author. | |
| 856 | 4 | 0 | |3 Taylor & Francis - Full text online |u http://www.library.illinois.edu/proxy/go.php?url=https://www.taylorfrancis.com/books/9781351021227 |
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| 655 | 4 | |a Electronic books. | |
| 776 | 0 | 8 | |z 9781351021210 |z 9781351021203 |
| 852 | 8 | |b eresour-nc |h Online Resource |t 1 |z Accessible anywhere on campus or with UIUC NetID | |
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| 952 | |0 0 |1 0 |2 ddc |4 0 |6 572_330000000000000_SEN_A |7 0 |9 395795 |a BIO |b BIO |c ST1 |d 2019-10-25 |e The Bookshop, Publication division, University of Calicut. |g 8528.50 |i 2357 |l 1 |o 572.33 SEN/ A |p BIO2357 |r 2021-01-08 |s 2020-10-20 |v 11525.00 |y BK |k Pound 125.00 | ||