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Protein chromatography : methods and protocols /
| Other Authors: | , |
|---|---|
| Format: | Printed Book |
| Language: | English |
| Published: |
New York, N.Y. :
Humana Press,
c2011.
|
| Series: | Springer protocols
Methods in molecular biology, v. 681 |
| Subjects: |
Table of Contents:
- A digest of protein purification / Dermot Walls, Robert McGrath, and Sinéad T. Loughran
- Gel-filtration chromatography / Ciaran Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor
- Immunoaffinity chromatography Jenny Fitzgerald ... [et al.]
- Avoiding proteolysis during protein chromatography / Barry J. Ryan
- Scale-up of protein purification : downstream processing issues / John Joseph Milne
- Phage display : a powerful technology for the generation of high specificity affinity reagents from alternative immune sources / William J.J. Finlay, Laird Bloom, and Orla Cunningham
- Engineering protein stability / Ciarán Ó'Fagáin
- Microfluidics in protein chromatography / Frank A. Gomez
- Tagging recombinant proteins to enhance solubility and aid purification / Dermot Walls and Sinéad T. Loughran
- Storage and lyophilisation of pure proteins / Ciarán Ó'Fagáin
- Differential precipitation and solubilization of proteins / Barry J. Ryan
- Ion-exchange chromatography : basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase / Philip M. Cummins, Oonagh Dowling, and Brendan F. O'Connor
- Protein quantitation and analysis of purity / Eva M. Campion, Sinéad T. Loughran, and Dermot Walls
- Purification of proteins fused to glutathione S-transferase / Sandra Harper and David W. Speicher
- Purification of proteins fused to maltose-binding protein / Mario Lebendiker and Tsafi Danieli
- Purification of proteins from baculovirus-infected insect cells / Luke O'Shaughnessy and Sean Doyle
- Purification of poly-histidine-tagged proteins / Sinéad T. Loughran and Dermot Walls
- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by CID/ETD tandem mass spectrometry / Rosana Navajas, Alberto Paradela, and Juan Pablo Albar
- Tag removal by site-specific cleavage of recombinant fusion proteins / Adam Charlton and Michael Zachariou
- Purification of antibodies using affinity chromatography / Elaine Darcy ... [et al.]
- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens / William J.J. Finlay, Laird Bloom, and Orla Cunningham
- Measuring protein-protein interactions using Biacore / Paul Leonard, Stephen Hearty, and Richard O'Kennedy
- Ultra-performance liquid chromatography-mass spectrometry of proteins / Timothy R. Croley
- Hydrophobic interaction chromatography / Philip M. Cummins and Brendan F. O'Connor
- Fast protein liquid chromatography / Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan
- Shotgun proteomics : a qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS / Laurent Geiser ... [et al.]
- Shotgun proteomics : a relative quantitative approach using off-gel electrophoresis and LC-MS/MS / Laurent Geiser ... [et al.]
- Clinical proteomics : liquid chromatography-mass spectrometry purification systems / Michael Henry and Paula Meleady
- Strategies for the purification of membrane proteins / Sinead Marian Smith
- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases / Tracey McCarthy and Maria G. Tuohy.