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Protein chromatography : methods and protocols /
| Other Authors: | , |
|---|---|
| Format: | Printed Book |
| Language: | English |
| Published: |
New York, N.Y. :
Humana Press,
c2011.
|
| Series: | Springer protocols
Methods in molecular biology, v. 681 |
| Subjects: |
| LEADER | 04856cam a22003617a 4500 | ||
|---|---|---|---|
| 008 | 101006s2011 nyua ob 001 0 eng d | ||
| 999 | |c 332063 |d 332063 | ||
| 010 | |a 2010938718 | ||
| 020 | |a 9781607619123 (alk. paper) | ||
| 020 | |a 1607619121 (alk. paper) | ||
| 020 | |a 9781607619130 (eISBN) | ||
| 035 | |a (OCoLC)ocn680057439 | ||
| 042 | |a lccopycat | ||
| 060 | 4 | |a QU 55 |b P964145 2011 | |
| 245 | 0 | 0 | |a Protein chromatography : |b methods and protocols / |c edited by Dermot Walls and Sinéad T. Loughran. |
| 260 | |a New York, N.Y. : |b Humana Press, |c c2011. | ||
| 300 | |a x, 527 p. : |b ill. ; |c 27 cm. | ||
| 490 | 0 | |a Springer protocols | |
| 490 | 0 | |a Methods in molecular biology, |x 1940-6029 ; |v v. 681 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a A digest of protein purification / Dermot Walls, Robert McGrath, and Sinéad T. Loughran -- Gel-filtration chromatography / Ciaran Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor -- Immunoaffinity chromatography Jenny Fitzgerald ... [et al.] -- Avoiding proteolysis during protein chromatography / Barry J. Ryan -- Scale-up of protein purification : downstream processing issues / John Joseph Milne -- Phage display : a powerful technology for the generation of high specificity affinity reagents from alternative immune sources / William J.J. Finlay, Laird Bloom, and Orla Cunningham -- Engineering protein stability / Ciarán Ó'Fagáin -- Microfluidics in protein chromatography / Frank A. Gomez -- Tagging recombinant proteins to enhance solubility and aid purification / Dermot Walls and Sinéad T. Loughran -- Storage and lyophilisation of pure proteins / Ciarán Ó'Fagáin -- Differential precipitation and solubilization of proteins / Barry J. Ryan -- Ion-exchange chromatography : basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase / Philip M. Cummins, Oonagh Dowling, and Brendan F. O'Connor -- Protein quantitation and analysis of purity / Eva M. Campion, Sinéad T. Loughran, and Dermot Walls -- Purification of proteins fused to glutathione S-transferase / Sandra Harper and David W. Speicher -- Purification of proteins fused to maltose-binding protein / Mario Lebendiker and Tsafi Danieli -- Purification of proteins from baculovirus-infected insect cells / Luke O'Shaughnessy and Sean Doyle -- Purification of poly-histidine-tagged proteins / Sinéad T. Loughran and Dermot Walls -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by CID/ETD tandem mass spectrometry / Rosana Navajas, Alberto Paradela, and Juan Pablo Albar -- Tag removal by site-specific cleavage of recombinant fusion proteins / Adam Charlton and Michael Zachariou -- Purification of antibodies using affinity chromatography / Elaine Darcy ... [et al.] -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens / William J.J. Finlay, Laird Bloom, and Orla Cunningham -- Measuring protein-protein interactions using Biacore / Paul Leonard, Stephen Hearty, and Richard O'Kennedy -- Ultra-performance liquid chromatography-mass spectrometry of proteins / Timothy R. Croley -- Hydrophobic interaction chromatography / Philip M. Cummins and Brendan F. O'Connor -- Fast protein liquid chromatography / Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan -- Shotgun proteomics : a qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS / Laurent Geiser ... [et al.] -- Shotgun proteomics : a relative quantitative approach using off-gel electrophoresis and LC-MS/MS / Laurent Geiser ... [et al.] -- Clinical proteomics : liquid chromatography-mass spectrometry purification systems / Michael Henry and Paula Meleady -- Strategies for the purification of membrane proteins / Sinead Marian Smith -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases / Tracey McCarthy and Maria G. Tuohy. | |
| 650 | 0 | |a Proteomics. | |
| 650 | 0 | |a Proteins |x Analysis. | |
| 650 | 0 | |a Chromatographic analysis. | |
| 650 | 1 | 2 | |a Proteins |x analysis |v Laboratory Manuals. |
| 650 | 1 | 2 | |a Chromatography |x methods |v Laboratory Manuals. |
| 700 | 1 | |a Walls, Dermot. | |
| 700 | 1 | |a Loughran, Sinéad T. | |
| 942 | |c BK | ||
| 776 | 0 | 8 | |i Online version: |t Protein chromatography. |d New York, N.Y. : Humana Press, c2011 |z 9781607619130 |w (OCoLC)676859157 |
| 906 | |a 7 |b cbc |c copycat |d 2 |e epcn |f 20 |g y-gencatlg | ||
| 955 | |a pc17 2010-10-06 |a xh00 2011-06-06 to USPL/STM |a xh00 2011-12-21 to USPL/STM |b th57 2012-07-17 z-processor to US ANGLO |i hc05 2012-09-26 to BCCD, c. 1 | ||
| 952 | |0 0 |1 0 |2 ddc |4 0 |6 572_600000000000000_PRO |7 0 |9 378787 |a BIO |b BIO |c ST1 |d 2019-01-11 |e The Bookshop, Publication division, University of Calicut. |g 9990.47 |i 2295 |l 0 |o 572.6 PRO |p BIO2295 |r 2019-02-25 |v 13875.66 |y BK |k EURO 164.99 | ||