Table of Contents:
  • Expression strategy / Michael R. Dyson
  • Protein expression in Escherichia coli / Rosalind Kim
  • Expression engineering of synthetic antibodies using ribosome display / Lydia M. Contreras Martinez and Matthew P. DeLisa
  • Refolding proteins from inclusion bodies / Renaud Vincentelli
  • Selection of protein variants with improved expression using green fluorescent protein-derived folding and solubility reporters / St�ephanie Cabantous and Geoffrey S. Waldo
  • Protein expression in the wheat-germ cell-free system / Tatsuya Sawasaki and Yaeta Endo
  • Saccharomyces cerevisiae : a microbial eukaryotic expression system / Christine Lang
  • Expression of proteins in Pichia pastoris / Geoff P. Lin-Cereghino, Wilson Leung, and Joan Lin-Cereghino
  • Improved baculovirus expression vectors / Richard B. Hitchman, Robert D. Possee, and Linda A. King
  • Transient transfection of insect cells for rapid expression screening and protein production / Kathryn H. Loomis [and others]
  • Generation of stable CHO cell lines for protein expression / Zhijian Lu [and others]
  • Transient expression in HEK293-EBNA1 cells / Roseanne Tom, Louis Bisson, and Yves Durocher
  • Nisin- and subtilin-controlled gene expression systems for gram-positive bacteria / Oscar P. Kuipers and Jan Kok
  • Protein production using lentiviral vectors / R�enald Gilbert, Sophie Broussau, and Bernard Massie
  • Expression in mammalian cells using BacMam viruses / Hsiao-Ping Lee and Yu-Chen Hu.