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Protein purification : principles, high resolution methods, and applications /

Bibliographic Details
Other Authors: Janson, Jan-Christer
Format: Printed Book
Language:English
Published: Hoboken, N.J. : John Wiley & Sons, c2011.
Edition:3rd ed.
Series:Methods of biochemical analysis ; v. 54.
Subjects:
Table of Contents:
  • Introduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson
  • Introduction to chromatography / J.-C. Janson and J.Å. Jönsson
  • Gel filtration: Size exclusion chromatography / L. Hagel
  • Ion exchange chromatography / E. Karlsson and I. Hirsh
  • High-resolution reversed-phase chromatography of proteins / S.W. Pettersson
  • Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew
  • Immobilized metal ion affinity chromatography / L. Kågedal
  • Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson
  • Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson
  • Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann
  • Affinity ligands from biological combinatorial libraries / P.-Å. Nygren
  • Membrane separations / J.K. Walter ... [et al.]
  • Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu
  • Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine
  • Electrophoresis in gels / R. Westermeier
  • Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti
  • Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg
  • Protein elution and blotting techniques / R. Westermeier
  • Capillary electrophoretic separations / W. Thormann
  • High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.