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A laboratory guide to genomic sequencing : the direct sequencing of native uncloned DNA /
A Safety Considerations Genomic sequencing involves a number of hazardous steps, such as high current, high voltage, radioactive and highly toxic chemicals. It is, therefore, absolutelyessen- tial that the instructions of equipment manufacturers be followed and that particular attention is paid to t...
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| Format: | Printed Book |
| Language: | English |
| Published: |
Basel:
Birkhauser,
1987.
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| LEADER | 01608nam a22001817a 4500 | ||
|---|---|---|---|
| 008 | 140426b xxu||||| |||| 00| 0 eng d | ||
| 020 | |a 3-7643-1925-9 | ||
| 020 | |a 9780817619251 | ||
| 082 | |a 574.87322 | ||
| 100 | |a Saluz, Hanspeter | ||
| 245 | |a A laboratory guide to genomic sequencing : |b the direct sequencing of native uncloned DNA / |c Hanspeter Saluz and J P Jost | ||
| 260 | |a Basel: |b Birkhauser, |c 1987. | ||
| 300 | |a 276p. : |c 27 cm. | ||
| 520 | |a A Safety Considerations Genomic sequencing involves a number of hazardous steps, such as high current, high voltage, radioactive and highly toxic chemicals. It is, therefore, absolutelyessen- tial that the instructions of equipment manufacturers be followed and that particular attention is paid to the local and federal safety regulations. INTRODUCTION 9 B Introduction During the cloning of genomic DNA many of its characteristics are perma- nently lost. It was therefore necessary to develop a new technique that would give us a closer look at a gene in its normal environment. The powerful technique of genomic sequencing, first described by Church and Gilbert (1984) now makes it possible to have a precise view of a given DNA sequence in a chromosome. This method combines the chemical DNA-sequencing procedure of Maxam and Gilbert (1980) with the detection of DNA sequences by electroblotting and indirect end-labeling by hybridization | ||
| 700 | |a J P Jost | ||
| 942 | |2 ddc |c BK | ||
| 999 | |c 151113 |d 151113 | ||
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