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Protein sequencing protocols /
| Other Authors: | |
|---|---|
| Format: | Printed Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
c2002.
|
| Edition: | 2nd ed. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 211. |
| Subjects: | |
| Online Access: | http://www.loc.gov/catdir/toc/fy033/2002022740.html http://www.loc.gov/catdir/enhancements/fy0825/2002022740-d.html |
Table of Contents:
- Machine generated contents note: Preface
- Contributors
- 1 Strategies for Handling Polypeptides on a Micro-Scale
- Bryan John Smith and Paul Tempst
- 2 SDS Polyacrylamide Gel Electrophoresis
- for N-Terminal Protein Sequencing
- Bryan John Smith
- 3 Two-Dimensional Polyacrylamide Gel Electrophoresis
- for the Separation of Proteins for Chemical Characterization
- Michael J. Dunn
- 4 Electroblotting of Proteins from Polyacrylamide Gels
- for Chemical Characterization
- Michael J. Dunn
- 5 Enzymatic Cleavage of Proteins
- Bryan John Smith
- 6 Chemical Cleavage of Polypeptides
- Bryan John Smith
- 7 Enzymatic Preparation and Isolation of Glycopeptides
- David J. Harvey
- 8 Analytical and Micropreparative Capillary Electrophoresis of Peptides
- Alan J. Smith
- 9 High-Performance Liquid Chromatography On-Line Derivative
- Spectroscopy for the Characterization of Peptides
- with Aromatic Amino Acid Residues
- Christoph W. Turck
- 10 Hydrolysis of Samples for Amino Acid Analysis
- lan Davidson
- 11 Amino Acid Analysis: Precolumn Derivatization Methods
- G. Brent Irvine and lan Davidson
- 12 Post Column Amino Acid Analysis
- Alan J. Smith
- 13 Amino Acid Analysis Using Pre-Column Derivatization
- with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate:
- Analysis of Hydrolyzed Proteins and Electroblotted Samples
- Steven A. Cohen
- 14 Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange
- Chromatography and IPAD Detection
- Petr Jandik, Jun Cheng, and Nebojsa Avdalovic
- 15 D-Amino Acid Analysis
- Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa
- 16 Validation of Amino Acid Analysis Methods
- Andrew J. Reason
- 17 Electrospray Mass Spectrometry of Peptides, Proteins
- and Glycoproteins
- Fiona M. Greer and Howard R. Morris
- 18 Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry
- Darryl J. C. Pappin
- 19 Nanoelectrospray Tandem Mass Spectrometry and Sequence
- Similarity Searching for Identification of Proteins
- from Organisms with Unknown Genomes
- Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork,
- and Andrej Shevchenko
- 20 Direct Identification of Proteins in Ultracomplex Mixtures:
- Applications to Proteome Analysis
- David M. Schieltz and John R. Yates, 111
- 21 Identification of PTH-Amino Acids by HPLC
- Gregory A. Grant and Mark W. Crankshaw
- 22 Protein Sequencer Maintenance and Troubleshooting
- Bryan Dunbar
- 23 N-Terminal Protein Sequencing for Special Applications
- Philip J. Jackson
- 24 Identification of Phosphorylation Sites by Edman Degradation
- Laurey Steinke and Richard G. Cook
- 25 Validation of Protein Sequencing in a Regulated Laboratory
- Philip J. Jackson and Neil Dodsworth
- 26 Automated C-Terminal Protein Sequence Analysis
- Using the Alkylated-Thiohydantoin Method
- David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham
- 27 Chemical Modifications of Proteins as an Aid to Sequence Analysis
- Alex F. Carne
- 28 Deblocking of N-Terminally Modified Proteins
- Hisashi Hirano and Roza M. Kamp
- 29 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid
- Jacek Mozdzanowski
- 30 Identification of Sites of Glycosylation
- David J. Harvey
- 31 Analysis of Sites of Protein Phosphorylation
- Alastair Aitken and Michele Learmonth
- 32 Quantitation and Location of Disulfide Bonds in Proteins
- Alastair Aitken and Michele Learmonth
- 33 Getting the Most from Your Protein Sequence
- Richard R. Copley and Robert B. Russell
- Appendix 1: Letter Codes, Structures, Masses, and Derivatives
- of Amino Acids
- James P. Turner
- Appendix 2: Protein Consensus Sequence Motifs
- Alastair Aitken.