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Protein sequencing protocols /
| Other Authors: | |
|---|---|
| Format: | Printed Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
c2002.
|
| Edition: | 2nd ed. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 211. |
| Subjects: | |
| Online Access: | http://www.loc.gov/catdir/toc/fy033/2002022740.html http://www.loc.gov/catdir/enhancements/fy0825/2002022740-d.html |
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| 010 | |a 2002022740 | ||
| 020 | |a 0896039757 (alk. paper) | ||
| 035 | |a (DLC) 2002022740 | ||
| 035 | |a (DLC)1291 | ||
| 042 | |a pcc | ||
| 082 | 0 | 0 | |a 572.8/633 |2 21 |
| 245 | 0 | 0 | |a Protein sequencing protocols / |c edited by Bryan John Smith. |
| 250 | |a 2nd ed. | ||
| 260 | |a Totowa, N.J. : |b Humana Press, |c c2002. | ||
| 300 | |a xii, 493 p. : |b ill. ; |c 23 cm. | ||
| 490 | 1 | |a Methods in molecular biology ; |v v. 211 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 8 | |a Machine generated contents note: Preface -- Contributors -- 1 Strategies for Handling Polypeptides on a Micro-Scale -- Bryan John Smith and Paul Tempst -- 2 SDS Polyacrylamide Gel Electrophoresis -- for N-Terminal Protein Sequencing -- Bryan John Smith -- 3 Two-Dimensional Polyacrylamide Gel Electrophoresis -- for the Separation of Proteins for Chemical Characterization -- Michael J. Dunn -- 4 Electroblotting of Proteins from Polyacrylamide Gels -- for Chemical Characterization -- Michael J. Dunn -- 5 Enzymatic Cleavage of Proteins -- Bryan John Smith -- 6 Chemical Cleavage of Polypeptides -- Bryan John Smith -- 7 Enzymatic Preparation and Isolation of Glycopeptides -- David J. Harvey -- 8 Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Alan J. Smith -- 9 High-Performance Liquid Chromatography On-Line Derivative -- Spectroscopy for the Characterization of Peptides -- with Aromatic Amino Acid Residues -- Christoph W. Turck -- 10 Hydrolysis of Samples for Amino Acid Analysis -- lan Davidson -- 11 Amino Acid Analysis: Precolumn Derivatization Methods -- G. Brent Irvine and lan Davidson -- 12 Post Column Amino Acid Analysis -- Alan J. Smith -- 13 Amino Acid Analysis Using Pre-Column Derivatization -- with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: -- Analysis of Hydrolyzed Proteins and Electroblotted Samples -- Steven A. Cohen -- 14 Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange -- Chromatography and IPAD Detection -- Petr Jandik, Jun Cheng, and Nebojsa Avdalovic -- 15 D-Amino Acid Analysis -- Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa -- 16 Validation of Amino Acid Analysis Methods -- Andrew J. Reason -- 17 Electrospray Mass Spectrometry of Peptides, Proteins -- and Glycoproteins -- Fiona M. Greer and Howard R. Morris -- 18 Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Darryl J. C. Pappin -- 19 Nanoelectrospray Tandem Mass Spectrometry and Sequence -- Similarity Searching for Identification of Proteins -- from Organisms with Unknown Genomes -- Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, -- and Andrej Shevchenko -- 20 Direct Identification of Proteins in Ultracomplex Mixtures: -- Applications to Proteome Analysis -- David M. Schieltz and John R. Yates, 111 -- 21 Identification of PTH-Amino Acids by HPLC -- Gregory A. Grant and Mark W. Crankshaw -- 22 Protein Sequencer Maintenance and Troubleshooting -- Bryan Dunbar -- 23 N-Terminal Protein Sequencing for Special Applications -- Philip J. Jackson -- 24 Identification of Phosphorylation Sites by Edman Degradation -- Laurey Steinke and Richard G. Cook -- 25 Validation of Protein Sequencing in a Regulated Laboratory -- Philip J. Jackson and Neil Dodsworth -- 26 Automated C-Terminal Protein Sequence Analysis -- Using the Alkylated-Thiohydantoin Method -- David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham -- 27 Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Alex F. Carne -- 28 Deblocking of N-Terminally Modified Proteins -- Hisashi Hirano and Roza M. Kamp -- 29 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid -- Jacek Mozdzanowski -- 30 Identification of Sites of Glycosylation -- David J. Harvey -- 31 Analysis of Sites of Protein Phosphorylation -- Alastair Aitken and Michele Learmonth -- 32 Quantitation and Location of Disulfide Bonds in Proteins -- Alastair Aitken and Michele Learmonth -- 33 Getting the Most from Your Protein Sequence -- Richard R. Copley and Robert B. Russell -- Appendix 1: Letter Codes, Structures, Masses, and Derivatives -- of Amino Acids -- James P. Turner -- Appendix 2: Protein Consensus Sequence Motifs -- Alastair Aitken. | |
| 650 | 0 | |a Nucleotide sequence |v Laboratory manuals. | |
| 700 | 1 | |a Smith, Bryan John. | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 211. | |
| 856 | 4 | 1 | |u http://www.loc.gov/catdir/toc/fy033/2002022740.html |
| 856 | 4 | 2 | |u http://www.loc.gov/catdir/enhancements/fy0825/2002022740-d.html |
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