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Antibodies : a laboratory manual /
"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibo...
| Other Authors: | |
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| Format: | Printed Book |
| Language: | English |
| Edition: | Second edition. |
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Table of Contents:
- Machine generated contents note:
- Preface
- 1 Antibody Production by the Immune System
- Stefanie Sarantopoulos
- 2 The Antibody Molecule
- Stefanie Sarantopoulos
- 3 Antibody-Antigen Interactions
- Stefanie Sarantopoulos
- 4 Antibody Responses
- Stefanie Sarantopoulos
- 5 Selecting the Antigen
- Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam
- 6 Immunizing Animals
- Edward A. Greenfield
- 7 Generating Monoclonal Antibodies
- Edward A. Greenfield
- 8 Growing Hybridomas
- Edward A. Greenfield
- 9 Characterizing Antibodies
- Frances Weis-Garcia and Robert H. Carnahan
- 10 Antibody Purification and Storage
- Jordan B. Fishman and Eric A. Berg
- 11 Engineering Antibodies
- James Dasch and Amy Dasch
- 12 Labeling Antibodies
- Eric A. Berg and Jordan B. Fishman
- 13 Immunoblotting
- Larisa Litovchick
- 14 Immunoprecipiation
- James DeCaprio and Thomas O. Kohl
- 15 Immunoassays
- Thomas O. Kohl and Carl A. Ascoli
- 16 Cell Staining
- Scott J. Rodig
- 17 Antibody Screening using High Throughput Flow Cytometry
- Thomas D.l. Duensing and Susan R. Watson
- Appendix I: Electrophoresis
- Appendix II: Protein Techniques
- Appendix III: General Information
- Appendix IV: Bacterial Expression
- Appendix V: Cautions
- Index.