Table of Contents:
  • Protein, proteome, and proteomics
  • Protein separation : 2D gel electrophoresis
  • Protein digestion
  • Peptide separation : HPLC
  • Fundamentals of mass spectrometry
  • Mass spectrometry : MALDI-TOF
  • Protein identification and characterization by MS
  • Tandem MS or MS/MS analysis
  • Fragmentation models
  • Identification and characterization by MS/MS
  • Spectral comparisons
  • Sequential comparison : de novo sequencing
  • Database searching for de novo sequences
  • Large-scale proteomics
  • Quantitative MS-based proteomics
  • Peptides to proteins
  • Top-down proteomics
  • Standards.