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High throughput protein expression and purification : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Printed Book |
| Language: | English |
| Published: |
Totowa, N.J. : [London :
Humana Press ; Springer, distributor],
c2009.
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
498. |
| Subjects: | |
| Online Access: | http://www.columbia.edu/cgi-bin/cul/resolve?clio8794065 |
Table of Contents:
- High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt
- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley
- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath
- Flexi vector cloning / Paul G. Blommel ... [et al.]
- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens
- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley
- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.]
- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart
- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery
- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
- Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh
- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood
- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina
- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.]
- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee
- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens
- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi
- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.]
- Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh
- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery.