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03936cam a22003494a 4500 |
| 006 |
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cr n |
| 008 |
080616s2009 njua sb 001 0 eng d |
| 020 |
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|a 9781588298799 (hbk. : alk. paper)
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| 082 |
0 |
4 |
|a 572.6
|2 22
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| 245 |
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|a High throughput protein expression and purification :
|b methods and protocols /
|c edited by Sharon A. Doyle.
|
| 260 |
|
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|a Totowa, N.J. :
|b Humana Press ;
|a [London :
|b Springer, distributor],
|c c2009.
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| 300 |
|
|
|a xii, 322p. ;
|c 27 cm.
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| 490 |
1 |
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|a Methods in molecular biology ;
|v 498
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| 504 |
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|a Includes bibliographical references and index.
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| 505 |
0 |
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|a High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... [et al.] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.] -- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
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| 505 |
0 |
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|a Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.] -- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.] -- Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery.
|
| 650 |
|
0 |
|a Proteins
|x Separation
|x Methodology.
|
| 650 |
1 |
2 |
|a Proteins
|x genetics.
|
| 650 |
1 |
2 |
|a Proteins
|x isolation & purification.
|
| 650 |
2 |
2 |
|a Gene Expression.
|
| 650 |
2 |
2 |
|a Proteomics
|x methods.
|
| 700 |
1 |
|
|a Doyle, Sharon A.
|e editor.
|
| 856 |
4 |
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|u http://www.columbia.edu/cgi-bin/cul/resolve?clio8794065
|
| 942 |
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|c BK
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| 210 |
1 |
0 |
|a High throughput protein expression and purification
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| 506 |
|
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|a License restrictions may limit access.
|
| 773 |
|
0 |
|t Springer Protocols
|
| 830 |
|
0 |
|a Methods in molecular biology (Clifton, N.J.) ;
|v 498.
|
| 910 |
|
|
|a Library of Congress record
|
| 999 |
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|c 101695
|d 101695
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