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DNA sequencing III : dealing with difficult templates /
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| Format: | Printed Book |
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Massachusetts:
Jones and Bartlett Publishers,
2009.
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Table of Contents:
- Controlled heat-denaturation of DNA plasmids and PCR fragments
- Heat denaturation is an effective step in sequencing of many difficult DNA templates
- Solutions for sequencing difficult regions
- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit
- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins
- Transcriptional sequencing as a tool for reading difficult-to-read templates
- Bias-free cloning of "unclonable" DNA for simplified genomic finishing
- Bioinformatics tools to aide sequencing of difficult templates.