LEADER 03399cam a22002777a 4500
020 |a 9781617797699 (alk. paper) 
082 0 0 |a 579.562  |b Q2 
100 |a Bill, Rosylin M  |9 17019 
245 0 0 |a Recombinant protein production in yeast :  |b methods and protocols /  |c ed by Roslyn M. Bill. 
260 |a New York:  |b Humana Press,  |c 2012. 
300 |a xii, 248 p. :  |b ill. (some col.) ; 
490 1 |a Methods in molecular biology 
490 1 |a Springer protocols 
504 |a Includes bibliographical references and index. 
505 0 |a Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby ... [et al.] -- Preparation of Pichia pastoris expression plasmids / Christel Logez ... [et al.] -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh ... [et al.] -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill -- Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare -- The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review) / Nagamani Bora ... [et al.] -- Online analysis and process control in recombinant protein production (review) / Shane M. Palmer and Edmund R.S. Kunji -- Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji -- Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji -- Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby -- Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim -- Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh ... [et al.] -- Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora -- Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby. 
650 0 |a Recombinant proteins-Laboratory manuals  |9 17020 
650 0 |a Yeast fungi-Biotechnology-Laboratory manuals  |9 17021 
650 1 2 |a Recombinant proteins-Biosynthesis-Laboratory Manuals.  |9 17022 
650 1 2 |a Yeasts-Laboratory Manuals  |9 17023 
650 2 2 |a Pichia-Laboratory Manuals  |9 17024 
650 2 2 |a Saccharomyces cerevisiae-Laboratory Manuals  |9 17025 
700 1 |a Bill, Roslyn M, Ed.  |9 17026 
856 |u http://link.springer.com/book/10.1007%2F978-1-61779-770-5 
942 |c BK 
999 |c 62593  |d 62593 
952 |0 0  |1 0  |4 0  |6 579_562000000000000_Q2  |7 0  |9 54410  |a MGUL  |b MGUL  |c GEN  |d 2014-02-20  |e Current Bill No 001352 dated 15.02.2014  |g 8488.53  |l 0  |o 579.562 Q2  |p 51004  |r 2014-09-24  |y BK