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Recombinant protein production in yeast : methods and protocols /
| Main Author: | |
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| Other Authors: | |
| Format: | Printed Book |
| Published: |
New York:
Humana Press,
2012.
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| Series: | Methods in molecular biology
Springer protocols |
| Subjects: | |
| Online Access: | http://link.springer.com/book/10.1007%2F978-1-61779-770-5 |
| LEADER | 03399cam a22002777a 4500 | ||
|---|---|---|---|
| 020 | |a 9781617797699 (alk. paper) | ||
| 082 | 0 | 0 | |a 579.562 |b Q2 |
| 100 | |a Bill, Rosylin M |9 17019 | ||
| 245 | 0 | 0 | |a Recombinant protein production in yeast : |b methods and protocols / |c ed by Roslyn M. Bill. |
| 260 | |a New York: |b Humana Press, |c 2012. | ||
| 300 | |a xii, 248 p. : |b ill. (some col.) ; | ||
| 490 | 1 | |a Methods in molecular biology | |
| 490 | 1 | |a Springer protocols | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby ... [et al.] -- Preparation of Pichia pastoris expression plasmids / Christel Logez ... [et al.] -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh ... [et al.] -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill -- Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare -- The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review) / Nagamani Bora ... [et al.] -- Online analysis and process control in recombinant protein production (review) / Shane M. Palmer and Edmund R.S. Kunji -- Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji -- Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji -- Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby -- Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim -- Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh ... [et al.] -- Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora -- Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby. | |
| 650 | 0 | |a Recombinant proteins-Laboratory manuals |9 17020 | |
| 650 | 0 | |a Yeast fungi-Biotechnology-Laboratory manuals |9 17021 | |
| 650 | 1 | 2 | |a Recombinant proteins-Biosynthesis-Laboratory Manuals. |9 17022 |
| 650 | 1 | 2 | |a Yeasts-Laboratory Manuals |9 17023 |
| 650 | 2 | 2 | |a Pichia-Laboratory Manuals |9 17024 |
| 650 | 2 | 2 | |a Saccharomyces cerevisiae-Laboratory Manuals |9 17025 |
| 700 | 1 | |a Bill, Roslyn M, Ed. |9 17026 | |
| 856 | |u http://link.springer.com/book/10.1007%2F978-1-61779-770-5 | ||
| 942 | |c BK | ||
| 999 | |c 62593 |d 62593 | ||
| 952 | |0 0 |1 0 |4 0 |6 579_562000000000000_Q2 |7 0 |9 54410 |a MGUL |b MGUL |c GEN |d 2014-02-20 |e Current Bill No 001352 dated 15.02.2014 |g 8488.53 |l 0 |o 579.562 Q2 |p 51004 |r 2014-09-24 |y BK | ||