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Cancer cell culture : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Printed Book |
| Published: |
Totowa:
Humana Press,
2011.
|
| Edition: | 2nd ed. |
| Series: | Methods in molecular biology,
Springer protocols |
| Subjects: | |
| Online Access: | http://scrc-en.tbzmed.ac.ir/uploads/91/CMS/user/file/197/books/Cancer%20cell%20culture.pdf |
Table of Contents:
- Xenotransplantation of breast cancers
- Feeder layers: Co-culture with nonneoplastic cells
- Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay
- Transfection and DNA-mediated gene transfer
- Analysis of drug interactions
- Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy
- Proteomic evaluation of cancer cells: Identification of cell surface proteins
- Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction
- Expression analysis of homeobox genes in leukemia/lymphoma cell lines
- Flow cytometric DNA analysis of human cancers and cell lines
- Angiogenesis assays
- Cell migration and invasion assays
- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR
- Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay
- Purification of annexin V and its use in the detection of apoptotic cells
- Real-time cytotoxicity assays
- Differential staining cytotoxicity assay: A review
- Cell sensitivity assays: The ATP-based tumor chemosensitivity assay
- Cell sensitivity assays: The MTT assay
- Cellular chemosensitivity assays: An overview
- Isolation of endothelial cells from human tumors
- Isolation of inflammatory cells from human tumours
- Establishment and culture of leukemia-lymphoma cell lines
- Isolation and culture of ovarian cancer cells and cell lines
- Isolation and culture of squamous cell carcinoma lines
- Isolation and culture of melanoma and naevus cells and cell lines
- Isolation and culture of colon cancer cells and cell lines
- Generation of lung cancer cell line variants by drug selection or cloning
- Quality assurance and good laboratory practice
- Elimination of mycoplasmas from infected cell lines using antibiotics
- Detecting mycoplasma contamination in cell cultures by polymerase chain reaction
- Cell culture contamination
- Cytogenetic analysis of cancer cell lines
- Online verification of human cell line identity by STRDNA typing
- Characterization and authentication of cancer cell lines: An overview
- Storage of cell lines
- Principles of cancer cell culture
- Cancer biology.