LEADER 03446cam a22002417a 4500
020 |a 9781617790799  
082 0 0 |a 616.994 07  |b Q1 
245 0 0 |a Cancer cell culture :  |b methods and protocols /  |c ed by Ian A. Cree. 
250 |a 2nd ed. 
260 |a Totowa:  |b Humana Press,  |c 2011. 
300 |a xiv, 502 p. :  |b ill. (some col.) ; 
490 1 |a Methods in molecular biology, 
490 1 |a Springer protocols 
504 |a Includes bibliographical references and index. 
505 0 |a Xenotransplantation of breast cancers -- Feeder layers: Co-culture with nonneoplastic cells -- Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay -- Transfection and DNA-mediated gene transfer -- Analysis of drug interactions -- Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy -- Proteomic evaluation of cancer cells: Identification of cell surface proteins -- Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction -- Expression analysis of homeobox genes in leukemia/lymphoma cell lines -- Flow cytometric DNA analysis of human cancers and cell lines -- Angiogenesis assays -- Cell migration and invasion assays -- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR -- Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay -- Purification of annexin V and its use in the detection of apoptotic cells -- Real-time cytotoxicity assays -- Differential staining cytotoxicity assay: A review -- Cell sensitivity assays: The ATP-based tumor chemosensitivity assay -- Cell sensitivity assays: The MTT assay -- Cellular chemosensitivity assays: An overview -- Isolation of endothelial cells from human tumors -- Isolation of inflammatory cells from human tumours -- Establishment and culture of leukemia-lymphoma cell lines -- Isolation and culture of ovarian cancer cells and cell lines -- Isolation and culture of squamous cell carcinoma lines -- Isolation and culture of melanoma and naevus cells and cell lines -- Isolation and culture of colon cancer cells and cell lines -- Generation of lung cancer cell line variants by drug selection or cloning -- Quality assurance and good laboratory practice -- Elimination of mycoplasmas from infected cell lines using antibiotics -- Detecting mycoplasma contamination in cell cultures by polymerase chain reaction -- Cell culture contamination -- Cytogenetic analysis of cancer cell lines -- Online verification of human cell line identity by STRDNA typing -- Characterization and authentication of cancer cell lines: An overview -- Storage of cell lines -- Principles of cancer cell culture -- Cancer biology. 
650 0 |a Cancer cells-Laboratory manuals.  |9 16578 
650 1 2 |a Tumor Cells, Cultured-Laboratory manuals.  |9 16579 
650 2 2 |a Laboratory Techniques and Procedures-Laboratory manuals.  |9 16580 
700 1 |a Cree, Ian A, Ed  |9 16529 
856 |u http://scrc-en.tbzmed.ac.ir/uploads/91/CMS/user/file/197/books/Cancer%20cell%20culture.pdf 
942 |c BK 
999 |c 62478  |d 62478 
952 |0 0  |1 0  |4 0  |6 616_994000000000000_07_Q1  |7 0  |9 54284  |a MGUL  |b MGUL  |c GEN  |d 2014-02-20  |e Current books Bill No 1352 dated 15.02.2014  |g 10727.11  |l 4  |m 1  |o 616.994 07 Q1  |p 50951  |r 2016-08-18  |s 2016-07-30  |y BK