Methods in alcohol-related neuroscience research /

Offers an overview of the many areas in investigating the effects of alcohol on the brain. This book provides technical guidance for investigators doing research at the molecular, cellular, systems, and behavioral levels.

Bibliographic Details
Main Author: Liu, Yuan; Ed. Lovinger, David M; Ed
Other Authors: Liu, Yuan, Lovinger, David M.
Format: Printed Book
Published: Boca Raton : CRC Press, [2002].
Series:Methods & new frontiers in neuroscience
Subjects:
Alcohol
Brain
Alcoholism
Alcohol-Induced Disorders, Nervous System
Neurology
Research
Online Access:http://www.loc.gov/catdir/toc/fy022/2001052454.html
http://www.loc.gov/catdir/enhancements/fy0646/2001052454-d.html
Table of Contents:
  • Machine generated contents note: Chapter 1 Quantitative Trait Locus (QTL) Mapping in Mice1
  • Abraham A. Palmer and Tamara J. Phillips
  • Chapter 2 Knockout and Knockin Mice 31
  • Gregg E. Homanics
  • Appendix 2.1 Preparation of Mouse Embryonic Fibroblast Feeder Cells 57
  • Appendix 2.2 ES Cell Transformation by Electroporation 59
  • Chapter 3 Molecular Studies by Chimeragenesis and Mutagenesis 63
  • Michael J. Beckstead, Gregory E Lopreato, Kent E. Vrana and S. John Mihic
  • Chapter 4 Combining Patch-Clamp Recording and Gene Profiling
  • in Single N eurons83
  • Hermes H. Yeh, Stavros Therianos and Shao-Ming Lu
  • Appendix 4.1 Protocol for Single-Cell mRNA Amplification/Reverse
  • N orthern A nalysis99
  • Appendix 4.2 Protocol for Single-Cell RT-PCR 109
  • Appendix 4.3 Protocol for Single-Cell Multiplex RT-PCR 111
  • Chapter 5 Quantitative Analysis of Neurotransmitter Receptor
  • Expression with Antibody-Based Techniques 113
  • C. Fernando Valenzuela and Daniel D. Savage
  • Appendix 5.1 Detailed Protocols for Western Immunoblotting139
  • Chapter 6 Artificial Bilayer Techniques in Ion Channel Study143
  • Steven N. Treistman, Robert O'Connell and John Crowley
  • Chapter 7 Rapid Drug Superfusion and Kinetic Analysis159
  • David M. Lovinger, Suzanne Sikes and Qing Zhou
  • Chapter 8 Electrophysiological Assessment of Synaptic
  • Transmission in Brain Slices 191
  • Jeff L. Weiner
  • Chapter 9 Cognitive Correlates of Single Neuron Activity
  • in Task-Performing Animals 219
  • Bennet Givens and T Michael Gill
  • Chapter 10 Multichannel Neural Ensemble Recording During
  • Alcohol Self-Administration241
  • Patricia H. Janak
  • Chapter 11 Combined Single-Neuron Recording and Microdialysis
  • in the Brain of Freely Behaving Animals (Rodents
  • and Non-Human Primates) 259
  • Nandor Ludvig
  • Chapter 12 Quantitative Microdialysis for in vivo Studies
  • of Pharmacodynamics 287
  • Rueben A. Gonzales, Amanda Tang and Donita L. Robinson
  • Chapter 13 Structural Magnetic Resonance Imaging (MRI)
  • of the Human Brain 319
  • Terry L. Jerigan
  • Chapter 14 Biochemical, Functional and Microstructural
  • Magnetic Resonance Imaging (MRI) 345
  • Elfar Adalsteinsson, Edith V. Sullivan and Adolf Pfefferbaum
  • Index 373.

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