Basic methods in protein purification and analysis

Bibliographic Details
Main Author: Simpson, Richard J.
Other Authors: Adams, Peter D., Golemis, Erica
Format: Printed Book
Published: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2009.
Subjects:
Proteins
Chromatography
Electrophoresis
Immunoprecipitation
Online Access:http://www.loc.gov/catdir/toc/fy0903/2008031690.html
Table of Contents:
  • Introduction to protein purification strategies
  • Preparation of cellular and subcellular extracts
  • One-dimensional polyacrylamide gel electrophoresis
  • Preparative two-dimensional gel electrophoresis with immobilized pH gradients
  • Ion-exchange chromatography
  • Size-exclusion chromatography
  • Reversed-phase high-performance liquid chromatography
  • Affinity and immunoaffinity chromatography
  • Metal chelate affinity chromatography
  • Multidimensional chromatography of intact proteins
  • Identification of protein-protein interactions by conventional column chromatography
  • Identification of associated proteins by coimmunoprecipitation
  • Chromatin immunoprecipitation of protein complexes
  • Identification of protein-protein interactions with glutathione-s-transferase fusion proteins
  • Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
  • In silico tools : analysis for creating focused interaction networks.
  • Introduction to protein purification strategies
  • Preparation of cellular and subcellular extracts
  • One-dimensional polyacrylamide gel electrophoresis
  • Preparative two-dimensional gel electrophoresis with immobilized PH gradients
  • Ion-exchange chromatography
  • Size-exclusion chromatography
  • Reversed-phase high-performance liquid chromatography
  • Affinity and immunoaffinity chromatography
  • Metal chelate affinity chromatography
  • Multidimensional chromatography of intact proteins
  • Identification of protein-protein interactions by conventional column chromatography
  • Identification of associated proteins by coimmunoprecipitation
  • Chromatin immunoprecipitation of protein complexes
  • Identification of protein-protein interactions with glutathione-s-transferase fusion proteins
  • Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
  • In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.

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