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| LEADER |
03027cam a22003014a 4500 |
| 020 |
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|a 9780879698683 (hardcover : alk. paper)
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| 020 |
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|a 9780879698676 (pbk. : alk. paper)
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| 082 |
0 |
0 |
|a 572.607 8
|b SIM/BA
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| 100 |
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|a Simpson, Richard J.
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| 245 |
0 |
0 |
|a Basic methods in protein purification and analysis
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| 260 |
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|a Cold Spring Harbor, N.Y. :
|b Cold Spring Harbor Laboratory Press,
|c c2009.
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| 300 |
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|a vii, 436 p. :
|b ill. ;
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| 505 |
0 |
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|a Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks.
|
| 505 |
0 |
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|a Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.
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| 650 |
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0 |
|a Proteins
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| 650 |
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0 |
|a Proteins
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| 650 |
1 |
2 |
|a Proteins
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| 650 |
2 |
2 |
|a Chromatography
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| 650 |
2 |
2 |
|a Electrophoresis
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| 650 |
2 |
2 |
|a Immunoprecipitation
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| 650 |
2 |
2 |
|a Proteins
|
| 700 |
1 |
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|a Simpson, Richard J.
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| 700 |
1 |
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|a Adams, Peter D.
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| 700 |
1 |
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|a Golemis, Erica.
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| 856 |
4 |
1 |
|u http://www.loc.gov/catdir/toc/fy0903/2008031690.html
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| 942 |
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|c BK
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|d 2016-05-04
|o 572.607 8 SIM/BA
|p 18061
|r 2016-05-04
|w 2016-05-04
|y BK
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