LEADER 05247cam a22003497a 4500
020 |a 9781588293787 (acidfree paper) 
020 |a 1588293785 (acidfree paper) 
082 0 0 |a 572.86  |b OST/MI 
100 |a Osterman/Andrei L 
245 0 0 |a Microbial gene essentiality 
260 |a Totowa, N.J. :  |b Humana Press,  |c c2008. 
300 |a 486  |b H B 
490 1 |a Methods in molecular biology ; 
505 0 |a Overview of whole-genome essentiality analysis / Karen Joy Shaw -- Pt. I. Experimental protocols. IA. Populational genome-wide essentiality screens. Transposon-based strategies for the identification of essential bacterial genes / William S. Reznikoff and Kelly M. Winterberg -- Identification and analysis of essential genes in Haemophilus influenzae / Sandy M.S. Wong and Brian J. Akerley -- Transposon site hybridization in Mycobacterium tuberculosis / Jeffrey P. Murry ... [et al.] -- Essential genes in the infection model of Pseudomonas aeruginosa PCR-based signature-tagged mutagenesis / François Sanschagrin, Irena Kukavica-Ibrulj, and Roger C. Levesque -- Whole-genome detection of conditionally essential and dispensable genes in Escherichia coli via genetic footprinting / Michael D. Scholle and Svetlana Y. Gerdes -- Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis / Taeok Bae ... [et al.] -- Multipurpose transposon insertion libraries for large-scale analysis of gene function in yeast / Anuj Kumar -- 
505 8 |a IB. Systematic collections of knockout mutants. How to make a defined near-saturation mutant library. Case 1: Pseudomonas aeruginosa PAO1 / Michael A. Jacobs -- Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentiality / Nicole T. Liberati ... [et al.] -- The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12 / Tomoya Baba and Hirotada Mori -- The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12 / Tomoya Baba ... [et al.] -- A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12 / Takeyoshi Miki, Yoshihiro Yamamoto, and Hideo Matsuda -- High-throughput creation of a whole-genome collection of yeast knockout strains / Angela M. Chu and Ronald W. Davis -- Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray / Pamela B. Meluh ... [et al.] -- 
505 8 |a IC. Genome minimization. Scarless engineering of the Escherichia coli genome / Tamás Fehér ... [et al.] -- Minimization of the Escherichia coli genome using the Tn5-targeted Cre/loxP excision system / Byung Jo Yu and Sun Chang Kim -- Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome / Jun-ichi Kato and Masayuki Hashimoto -- ID. Conditional knockouts. Identification of essential genes in Staphylococcus aureus by construction and screening of conditional mutant library / Dezhong Yin and Yinduo Ji -- Techniques for the isolation and use of conditionally expressed antisense RNA to achieve essential gene knockdowns in Staphylococcus aureus / Allyn Forsyth and Liangsu Wang -- Introduction of conditional lethal amber mutations in Escherichia coli / Christopher D. Herring -- Pt. II. Bioinformatics. IIA. Statistics. Statistical methods for building random transposon mutagenesis libraries / Oliver Will -- Statistical evaluation of genetic footprinting data / Gábor Balázsi -- Modeling competitive outgrowth of mutant populations : why do essentiality screens yield divergent results? / Alexander I. Grenov and Svetlana Y. Gerdes -- 
505 8 |a Statistical analysis of fitness data determined by TAG hybridization on microarrays / Brian D. Peyser, Rafael Irizarry, and Forrest A. Spencer -- IIB. Data integration and modeling. Profiling of Escherichia coli chromosome database / Yukiko Yamazaki, Hironori Niki, and Jun-ichi Kato -- Gene essentiality analysis based on DEG, a database of essential genes / Chun-Ting Zhang and Ren Zhang -- Detection of essential genes in Streptococcus pneumoniae using bioinformatics and allelic replacement mutagenesis / Jae-Hoon Song and Kwan Soo Ko -- Design and application of genome-scale reconstructed metabolic models / Isabel Rocha, Jochen Förster, and Jens Nielsen -- Predicting gene essentiality using genome-scale in silico models / Andrew R. Joyce and Bernhard Ø. Palsson -- Comparative approach to analysis of gene essentiality / Andrei L. Osterman and Svetlana Y. Gerdes. 
650 0 |a Microbial genetics. 
650 0 |a Computational biology. 
650 1 2 |a Genes, Essential 
650 1 2 |a Genetics, Microbial 
650 2 2 |a Genome, Bacterial 
650 2 2 |a Computational Biology 
650 2 2 |a Genomics 
650 2 2 |a Genetic Techniques 
650 2 2 |a Microbiological Techniques 
700 1 |a Osterman, Andrei L. 
700 1 |a Gerdes, Svetlana Y. 
856 4 0 |u http://www.columbia.edu/cgi-bin/cul/resolve?clio8804789 
942 |c BK 
999 |c 1796  |d 1796 
952 |0 0  |1 0  |4 0  |6 572_860000000000000_OST_MI  |7 0  |8 NFIC  |9 2652  |a KUFOS  |b KUFOS  |c GEN  |d 2016-05-03  |o 572.86 OST/MI  |p 17917  |r 2016-05-03  |w 2016-05-03  |y BK