Carregant...
Calcium techniques: a laboratory manual/
"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunctio...
| Altres autors: | , , , |
|---|---|
| Format: | Printed Book |
| Publicat: |
New York:
Cold spring harbor laboratory press,
2014.
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| Matèries: |
| LEADER | 01756cam a2200241 i 4500 | ||
|---|---|---|---|
| 020 | |a 9781936113583 | ||
| 082 | 0 | 0 | |a 572.516 |b CAL |
| 245 | 0 | 0 | |a Calcium techniques: a laboratory manual/ |
| 260 | |a New York: |b Cold spring harbor laboratory press, |c 2014. | ||
| 300 | |a xvi, 592 pages : |b illustrations (some color) ; | ||
| 505 | 0 | |a Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics. | |
| 520 | |a "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"-- | ||
| 650 | 1 | 2 | |a Calcium |
| 650 | 2 | 2 | |a Calcium Channels |
| 650 | 2 | 2 | |a Calcium Signaling |
| 650 | 2 | 2 | |a Fluorometry |
| 700 | 1 | |a Parys, Jan B., | |
| 700 | 1 | |a Bootman, Martin D., | |
| 700 | 1 | |a Yule, David I., | |
| 700 | 1 | |a Bultynck, Geert, | |
| 942 | |c BK | ||
| 999 | |c 53163 |d 53163 | ||
| 952 | |0 0 |1 0 |4 0 |6 572_516000000000000_CAL |7 0 |9 67280 |a KU |b KU |d 2019-02-08 |e PICO.A2/5736/2013 dtd 02/05/2018 |g 10995.00 |l 0 |o 572.516 CAL |p LS01561 |r 2019-02-08 |w 2019-02-08 |y BK | ||