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Seroprevalence Of Hydropericardium Syndrome In Broiler Chickens
In the present study, seroprevalence of hydropericardium syndrome (HPS) in broiler chickens in Kerala was evaluated. The results of agar gel precipitation test (AGPT), counter imrnunoelectrophoresis (eIE) and indirect enzyme linked imrnunoso...
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| Format: | Ph.D Thesis |
| Language: | Undetermined |
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Mannuthy
Department of Microbiology, College of Veterinary and Animal Sciences
2000
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| 245 | |a Seroprevalence Of Hydropericardium Syndrome In Broiler Chickens | ||
| 260 | |a Mannuthy |b Department of Microbiology, College of Veterinary and Animal Sciences |c 2000 | ||
| 502 | |b MVSc | ||
| 520 | 3 | |a In the present study, seroprevalence of hydropericardium syndrome (HPS) in broiler chickens in Kerala was evaluated. The results of agar gel precipitation test (AGPT), counter imrnunoelectrophoresis (eIE) and indirect enzyme linked imrnunosorbent assay (ELISA) were compared in detecting HPS antibodies. A total of 350 sera samples were collected from different parts of Kerala. In the first phase, 50 sera samples were screened by AGPT, eIE and indirect ELISA. Three samples were found positive by AGPT and eIE. Seven samples were found to be posi ti ve by indirect ELISA. Based on the comparison, another 300 samples were screened using indirect ELISA and 17 samples were found to be positive. A total of 24 samples were found positive for HPS antibodies out of 350 samples screened by indirect ELISA. The seroprevalence of 6.86 percentage was evaluated in broiler chickens in Kerala using indirect ELISA. The syndrome was experimentally reproduced in three-week-old broiler subcutaneous inoculation viz. , chicks. intramuscular, Various routes of and intraperitoneal routes were tried. The lesions were more pronounced when the birds were inoculated by subcutaneous route. Chick mortality was high on the fourth day. Electron microscopy of the infected liver homogenate revealed hexagonal virus particles of about 75 nm in size. These are suggestive of adenovirus. Histopathology of necropsied birds showed degeneration and necrosis of myocardial fibres, necrosis of hepatocytes and focal areas of coagulation in liver. Basophilic intranuclear inclusion bodies were demonstrated in hepatocytes. Vacuolar degeneration of kidney tubules, lymphoid depletion of bursa, vascular sclerosis of spleen and congestion and oedema of lungs were also seen. | |
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