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Cost- Effective Methods and Devices for home scale adoption of Plant Tissue Culture
Attempts were made to develop cost-effective methods and devices for home scale adoption of plant tissue culture in the plant tissue culture laboratory of the Department of Horticulture, College of Agriculture, Vellayani, during 1993-95. The test plant selected for the study was Anthurium andrea...
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| 格式: | Ph.D Thesis |
| 語言: | Undetermined |
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Vellayani
Department of Horticulture, College of Agriculture
1996
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| 總結: | Attempts were made to develop cost-effective methods and
devices for home scale adoption of plant tissue culture in the
plant tissue culture laboratory of the Department of Horticulture,
College of Agriculture, Vellayani, during 1993-95. The test plant
selected for the study was Anthurium andreanum Lind. (Pink).
Segments of leaf were used as explant for cullus initiation.
The callus so obtained was used for further regeneration studies.
Various low cost equipment were fabricated and tested for their
efficiency in comparison to the conventional expensive method.
One-fourth strength of the major nutrients of MS medium along with
full strength of micro nutrients was found good for the induction
of multiple shoots.
All the growth parameters were found adversely affected by the
use of LR grade chemicals, when compared to AR grade chemicals.
Confectionary grade sugar was found to be equally good to AR grade
sucrose, while commercial grade crystal sugar was not. Rain water
could be used as a substitute to double glass distilled water in
the culture medium. Attempts to substitute agar-agar with less
expensive playing marbles, as support matrix of the culture medium
was also successful.
Ordinary (gold-smith type) balance could be used to replace
the expensive electronic balance in weighing chemicals for media
preparation. The pH indicator paper could be effectively used
instead of the pH meter, in adjusting pH of the medium. Ordinary
colourless glass bottles and jam jars could be economically used,
instead of expensive borosilicate glassware.
The domestic pressure cooker was equally efficient as the
electric autoclave in sterilising culture medium and containers.
The expensive refrigerator could be effectively replaced with
ice-packed thermocol boxes. Instead of the laminar airflow
cabinet, the fabricated transfer hood could be effectively used.
Attempts to substitute artificial flourescent light with
natural light were successful. Rooted plantlets when planted out
exhibited 30 per cent loss during various stages of hardening.
The cost of producing a single anthurium plantlet was
Rs. 5.16 in the conventional method, whereas in the cost-effective
method it could be brought down to Rs. 1.82.
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