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DNA fingerprinting of promising selections of jack (Artocarpus heterophyllus Lam.) using molecular markers
The jack (Artocarpus heterophyllus Lam.) is the world’s largest edible fruit crop which belongs to the family Moraceae. It was originated from Western Ghats of India. India is one of the major jackfruit producing country. It is an important component in the homestead gardens of Kerala. In India, Ker...
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| Aineistotyyppi: | Ph.D Thesis |
| Julkaistu: |
Vellanikkara
Centre for Plant Biotechnology and Molecular Biology, College of Horticulture
2017
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| Aiheet: |
| LEADER | 04948nam a22001697a 4500 | ||
|---|---|---|---|
| 082 | |a 660.6 |b MAR/DN | ||
| 100 | |a Marjan P S | ||
| 245 | |a DNA fingerprinting of promising selections of jack (Artocarpus heterophyllus Lam.) using molecular markers | ||
| 260 | |a Vellanikkara |b Centre for Plant Biotechnology and Molecular Biology, College of Horticulture |c 2017 | ||
| 300 | |a 127p | ||
| 502 | |a MSc | ||
| 520 | 3 | |a The jack (Artocarpus heterophyllus Lam.) is the world’s largest edible fruit crop which belongs to the family Moraceae. It was originated from Western Ghats of India. India is one of the major jackfruit producing country. It is an important component in the homestead gardens of Kerala. In India, Kerala is the largest producer. Kerala has only a few numbers of varieties in jack. Muttom varikka is a popular cultivar of jackfruit in Kerala. RARS, Kumarakom had identified few superior jackfruit genotypes from Kuttanad region. Fingerprint data are not available for these selections/variety/cultivar. Central Seed Committee established under the Seed Act (1966) insists for DNA fingerprint data for the varieties released or proposed to be released. The study on “DNA fingerprinting of promising selections of Jack (Artocarpus heterophyllus Lam.) using molecular markers” was carried out at the Centre for Plant Biotechnology and Molecular Biology (CPBMB), Vellanikkara and Regional Agricultural Research Station, Kumarakom during 2015-17. The objective of present study was to characterize popular jack variety Sindhur, cultivar Muttom varikka and six superior jack selections (Veloor varikka-1, Veloor varikka-2, Pathamuttom varikka-1, Pathamuttom Varikka-2, Kavanar Varikka-1and Chengalam varikka) identified at RARS, Kumarakom from Kuttanad tract using SSR and ISSR markers. High variability was observed in qualitative, quantitative and biochemical characters of eight jack genotypes. The variety Sindhur and selections Pathamuttom varikka-1 and Pathamuttom varikka-2 showed coppery red colour for flake flesh which is a character having high market value. For molecular analysis, genomic DNA extraction was done using modified CTAB method (Doyle and Doyle, 1987). The RNA contamination was completely removed through RNase treatment. Good quality DNA with UV absorbance ratio (A260/A280) 1.80 - 1.90 was used for further analysis. Fifty each of ISSR and SSR primers were screened for amplification of jackfruit genomic DNA and those which showed reliable and distinct banding pattern were selected for further amplification and fingerprinting. Ten ISSR and eleven SSR primers were selected for developing fingerprints of jack varieties and promising selections. Only clear and distinct bands were used for developing DNA fingerprints. Specific fingerprints were developed by assigning different colour codes for unique and sharing bands. Unique bands were observed in Pathamuttom varikka -1, Pathamuttom varikka -2, Chengalam varikka, Muttom varikka and Sindhur with ISSR marker system. The SSR marker system also could bring out unique bands in Pathamuttom varikka-1, Pathamuttom varikka -2, Chengalam varikka and Sindhur. These specific bands could be utilized for the identification of varieties and selections. The Marker Index (MI) obtained for the selected ISSR primers ranged from 1.15 to 3.77 and for SSR primers it ranged from 0.60 to 2.7 which indicated the efficiency of primers selected. The Polymorphic Information Content (PIC) varied from 0.23 to 0.30 in ISSR analysis and 0.22 to 0.98 in SSR analysis. This is also an indication of suitability of primers. The computer package NTSYS-Pc was used for diversity analysis. Among the eight genotypes maximum diversity (44 %) was observed for the variety Sindhur. The varieties Veloor varikka-1 and Veloor varikka-2 collected from same locality indicated 95 percentage similarity. The number of clusters and distribution patterns were different in both quantitative data and combined ISSR-SSR data. This may be because the actual coding sequences for quantitative characters were not used for molecular clustering. Homology has to be worked out between expressed gene sequence and quantitative data for obtaining more precise result. The specific DNA fingerprints developed for the jack variety Sindhur, cultivar Muttom varikka and promising selections Pathamuttom varikka-1, Pathamuttom varikka-2, Kavanar varikka-1 and Chengalam varikka could be utilized for varietal identification and settling IPR issues. Since the fingerprints obtained for Veloor varikka-1 and Veloor varikka-2 were identical more primers have to be screened for developing specific fingerprints for these selections. | |
| 650 | |a Plant Biotechnology and Molecular Biology | ||
| 700 | |a Anu G Krishnan (Guide) | ||
| 942 | |2 ddc |c TH | ||
| 999 | |c 157980 |d 157980 | ||
| 952 | |0 0 |1 0 |4 0 |6 660_600000000000000_MARDN |7 1 |8 REF |9 157770 |a KAUCLV |b KAUCLV |c THESES |d 2017-12-27 |o 660.6 MAR/DN |p 174100 |r 2017-12-27 |w 2017-12-27 |y TH | ||